Abstract
Paraneoplastic sensory neuronopathy (PSN) has been shown to harbor characteristic
anti-neuronal autoantibody ‘anti-Hu’ in their sera and cerebrospinal fluid. Creation
of animal models exhibiting clinical or pathological features seen in PSN by means
of passive transfer of anti-Hu positive IgG has not been achieved. Although, anti-Hu
antibody was shown to induce neuronal cell lysis in vitro, this result has not been
reproduced so far. Since prominent T cell infiltration are seen in the central nervous
system and posterior spinal ganglion of the patients with anti-Hu syndrome, we studied
cytotoxic T cell (CTL) activity in peripheral mononuclear cells from a patient with
PSN harboring anti-Hu antibody. The activated CD8+ T cells from the patient’s venous
blood were shown to lyse her own fibroblasts which were incubated with interferon-γ
to induce HLA class I molecules on their surface and the recombinant HuD protein was
injected into the cells by microinjector. This is the first report showing the existence
of CTL in a patient with PSN.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of the Neurological SciencesAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Microinjection of a p21ras antibody into PC12 cells inhibit neurite outgrowth induced by nerve growth factor and basic fibroblast growth factor.Growth Factors. 1991; 4: 145-155
- Regression of small-cell lung carcinoma in patients with paraneoplastic neuronal antibodies.Lancet. 1993; 341: 21-22
- Sensory neuronopathy and small cell lung cancer: antineuronal antibody that also reacts with the tumor.Am J Med. 1986; 80: 45-52
- Effect of intraventricular injection of an anti-Purkinje cell antibody (anti-Yo) in a guinea pig model.J Neurol Sci. 1991; 106: 82-87
- Type Iia (‘anti-Hu’) antineuronal antibodies produce destruction of rat cerebellar granule neurons in vitro.Neurology. 1993; 43: 2049-2054
- Changes in LAK susceptibility of tumor cells as their MHC class I antigen expression levels regenerate after treatment at pH 3.0.Immunol Lett. 1995; 44: 7-11
- Nuclear localization of anti-Hu antibody is not associated with in vitro cytotoxicity.J Neuroimmunol. 1994; 55: 205-212
- Inhibitory MHC class I receptors on NK cells and T cells.Immunol Today. 1996; 17: 86-91
- Natural killer cell receptors and MHC class I interactions.Curr Opin Immunol. 1997; 9: 126-131
- The major histocompatibility complex.in: Paul W.E. Fundamental Immunology. Lippincott-Raven, Philadelphia1999: 263-285
- Induction of tumor-reactive CTL from peripheral blood and tumor-infiltrating lymphocytes of melanoma patients by in vitro stimulation with an immunodominant peptide of the human melanoma antigen MART-1.J Immunol. 1995; 154: 2257-2265
- HuD, a paraneoplastic encephalomyelitis antigen, contains RNA-binding domains and is homologous to Elav and Sex-lethal.Cell. 1991; 67: 325-333
- Passive transfer and active immunization with the recombinant leucine-zipper (Yo) protein as an attempt to establish an animal model of paraneoplastic cerebellar degeneration.J Neurol Sci. 1994; 127: 153-158
- HLA A24 in paraneoplastic cerebellar degeneration with anti-Yo antibody.Neurology. 1996; 47: 606-607
- Changes in anti-HuD antibody titers in the long-term course in paraneoplastic sensory neuropathy.Eur Neurol. 1997; 37: 122-123
Article info
Publication history
Accepted:
January 12,
1999
Received in revised form:
December 9,
1998
Received:
March 12,
1998
Identification
Copyright
© 1999 Elsevier Science B.V. Published by Elsevier Inc. All rights reserved.
