Abstract
Proinflammatory cytokines contribute to the regulation of the disease process in inflammatory
neuropathies. Cellular localisation of cytokine expression in CIDP nerve biopsies
should provide further insight into the pathogenic mechanisms of the disease and the
individual cells involved. In this study in situ hybridisation was used to determine
the exact localisation and identity of cells that express TNFα, IFNγ and IL-2 mRNA
within the CIDP nerve. Paraffin embedded and frozen sural nerve biopsies from three
acute phase CIDP patients were used for the study. Sections of these samples were
probed with digoxigenin labelled oligoprobes for TNFα, IFNγ and IL-2. The results
demonstrate localisation of cytokine expression to the inner rim of the perineurium,
epineurial and endoneurial blood vessels and infiltrating inflammatory cells. In addition
strong staining for TNFα mRNA was widespread in the endoneurium in areas consistent
with/suggestive of Schwann cells. Expression of cytokines in the perineurium and endoneurial
blood vessels may have pertinent implications with respect to the breakdown of the
blood nerve barrier associated with CIDP. In the very least the potential for an immunomodulatory
role may be ascribed to these cells.
Keywords
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Article info
Publication history
Accepted:
December 8,
1998
Received in revised form:
December 8,
1998
Received:
August 20,
1998
Identification
Copyright
© 1999 Elsevier Science B.V. Published by Elsevier Inc. All rights reserved.
