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Electrophysiological characterization of Nsc-34 cell line using Microelectrode Array

  • K.R. Sabitha
    Affiliations
    Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bengaluru 560 029, India
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  • D. Sanjay
    Affiliations
    Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bengaluru 560 029, India
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  • B. Savita
    Affiliations
    Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bengaluru 560 029, India
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  • T.R. Raju
    Affiliations
    Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bengaluru 560 029, India
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  • T.R. Laxmi
    Correspondence
    Corresponding author at: Department of Neurophysiology, Hosur Road, P.B. No. 2900, NIMHANS, Bengaluru 560 029, India.
    Affiliations
    Department of Neurophysiology, National Institute of Mental Health and Neurosciences (NIMHANS), Hosur Road, Bengaluru 560 029, India
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Published:September 21, 2016DOI:https://doi.org/10.1016/j.jns.2016.09.038

      Highlights

      • Electrophysiological properties of NSC-34 cell lines were studied in the present study.
      • NSC 34 cell line has the ability to discharge spontaneously and rhythmically.
      • Mean firing frequency revealed that NSC 34 cell lines have >2 distinct types of neuronal populations.
      • NSC-34 cell line has both principal and interneurons.

      Abstract

      Neurons communicate with each other through intricate network to evolve higher brain functions. The electrical activity of the neurons plays a crucial role in shaping the connectivity. With motor neurons being vulnerable to neurodegenerative diseases, understanding the electrophysiological properties of motor neurons is the need of the hour, in order to comprehend the impairment of connectivity in these diseases. NSC-34 cell line serves as an excellent model to study the properties of motor neurons as they express Choline acetyltransferase (ChAT). Although NSC-34 cell lines have been used to study the effect of various toxicological, neurotrophic and neuroprotective agents, the electrical activity of these cells has not been elucidated. In the current study, we have characterized the electrophysiological properties of NSC-34 cell lines using Micro-Electrode Array (MEA) as a tool. Based on the spike waveform, firing frequency, auto- and cross-correlogram analysis, we demonstrate that NSC-34 cell culture has >2 distinct types of neuronal population: principal excitatory neurons, putative interneurons and unclassified neurons. The presence of interneurons in the NSC-34 culture was characterized by increased expression of GAD-67 markers. Thus, finding an understanding of the electrophysiological properties of different population of neurons in NSC-34 cell line, will have multiple applications in the treatment of neurological disorders.

      Keywords

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