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Neuroprotective effects of methane-rich saline on experimental acute carbon monoxide toxicity

  • Author Footnotes
    1 These authors contributed equally to this work.
    Meihua Shen
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Biomedical Information Engineering, Ministry of Education, Institute of Mitochondrial Biology and Medicine, Xi'an Jiaotong University, School of Life Science and Technology, Xi'an 710049, PR China

    Department of Intensive Care Unit, Shanghai Provincial Corps Hospital, Chinese People's Armed Police Forces, 831 Hongxu Road, Shanghai 201103, PR China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Danfeng Fan
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Department of Hyperbaric Oxygen, Navy General Hospital, No. 6, Fucheng Road, Beijing 100048, PR China
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  • Yu Zang
    Affiliations
    Department of Neurology, The Affiliated Hospital of North China University of Science and Technology, No.57, Jianshe South Road, Tangshan, Hebei 063000, PR China
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  • Yan Chen
    Affiliations
    Department of Intensive Care Unit, Shanghai Provincial Corps Hospital, Chinese People's Armed Police Forces, 831 Hongxu Road, Shanghai 201103, PR China
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  • Kaimin Zhu
    Affiliations
    Department of Intensive Care Unit, Shanghai Provincial Corps Hospital, Chinese People's Armed Police Forces, 831 Hongxu Road, Shanghai 201103, PR China
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  • Zhonghai Cai
    Affiliations
    Department of Intensive Care Unit, Shanghai Provincial Corps Hospital, Chinese People's Armed Police Forces, 831 Hongxu Road, Shanghai 201103, PR China
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  • Yueqin Liu
    Affiliations
    Department of Intensive Care Unit, Shanghai Provincial Corps Hospital, Chinese People's Armed Police Forces, 831 Hongxu Road, Shanghai 201103, PR China
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  • Xuejun Sun
    Affiliations
    Department of Naval Aeromedicine, Faculty of Naval Medicine, Second Military Medical University, No.800, Xiangyin Road, Shanghai 200433, PR China
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  • Jiankang Liu
    Correspondence
    Corresponding authors.
    Affiliations
    Key Laboratory of Biomedical Information Engineering, Ministry of Education, Institute of Mitochondrial Biology and Medicine, Xi'an Jiaotong University, School of Life Science and Technology, Xi'an 710049, PR China
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  • Jianfeng Gong
    Correspondence
    Corresponding authors.
    Affiliations
    Department of General Surgery, The Sixth People's Hospital, Shanghai Jiao Tong University, No.600, Yishan Road, Shanghai 200233, PR China
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  • Author Footnotes
    1 These authors contributed equally to this work.
Published:August 27, 2016DOI:https://doi.org/10.1016/j.jns.2016.08.055

      Highlights

      • Methane-rich saline (MRS) can protect the brain against acute carbon monoxide (CO) poisoning induced injury.
      • MRS may attenuate the oxidative stress in the brain after acute CO poisoning
      • MRS may attenuate the inflammation in the brain after acute CO poisoning

      Abstract

      Background

      Methane has been reported to play a protective role in ischemia-reperfusion injury via anti-oxidation, anti-inflammatory and anti-apoptotic activities. This study was designed to determine the protective effects of methane-rich saline (MRS) on acute carbon monoxide (CO) poisoning.

      Methods

      A total of 36 male Sprague-Dawley rats were randomly divided into 3 groups: sham group, CO group and MRS group. Acute CO poisoning was induced by exposing rats to 1000 ppm CO in air for 40 min and then to 3000 ppm CO for an additional 20 min until they lost consciousness. MRS at 10 ml/kg was intraperitoneally administered at 0 h, 8 h and 16 h after CO exposure. Rats were sacrificed 24 h after CO exposure. Brains were collected for Nissl staining. The cortex and hippocampus were separated for the detections of malondialdehyde (MDA), 3-nitrotyrosine (3-NT), 8-hydroxydeoxyguanosine (8-OHdG), tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β), interleukin-6 (IL-6) and superoxide dismutase (SOD) activities.

      Results

      The results showed that MRS treatment improved neuronal injury, reduced MDA, 3-NT and 8-OHdG, and increased SOD activity of the hippocampus and cortex compared with normal saline-treated rats. In addition, MRS reduced the expression of TNF-α and IL-1β in the brain but had no effect on IL-6 expression.

      Conclusion

      These findings suggest that MRS may protect the brain against acute CO poisoning-induced injury via its anti-oxidative and anti-inflammatory activities.

      Abbreviations:

      ANOVA (analysis of variance), CO (carbon monoxide), COHb (carboxyhemoglobin), DNS (delayed neurological syndrome), HBO (hyperbaric oxygen), •OH (hydroxyl radical), IL-1β (interleukin 1 –β), IL-6 (interleukin-6), MRS (methane-rich saline), MDA (Malondialdehyde), NS (normal saline), 3-NT (3-nitrotyrosine), 8-OHdG (8-hydroxydeoxyguanosine), ROS (reactive oxygen species), SOD (superoxide dismutase), ONOO− (peroxynitrite), O2− (superoxide anion), TBA (thiobarbituric acid), TNF-α (tumor necrosis factor-α)

      Keywords

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