Highlights
- •The levels of PLA including PMA, PLyA and PNA and polymorphisms of SELP S290N and PSGL-1 M62I were measured.
- •The differences of PMA, PLyA and PNA among subtype patients were compared respectively.
- •We compared the genotypes and allele frequencies of SELP S290N and PSGL-1 M62I among subtype patients respectively.
- •The relationship between PLA and SELP S290N and PSGL-1 M62I polymorphisms was analyzed.
Abstract
Background
Platelet-leukocyte aggregations (PLA) play key roles in acute ischemic stroke (AIS)
process and they are formed by the combination of P-selectin (SELP) expressed on the
surface of the platelet membranes with P-selectin glycoprotein ligand-1(PSGL-1) expressed
on the surface of the leukocytes. There are genetic polymorphisms in SELP and PSGL-1.
We tested the differences in all kinds of PLA among subtypes of AIS and the association
of SELP S290N and PSGL-1 M62I polymorphism with AIS to assess the correlations of
PLA with SELP S290N and PSGL-1 M62I genetic polymorphisms.
Methods
One hundred and forty-eight patients with acute ischemic stroke, including thirty
patients with large artery atherosclerosis stroke (LAA), twenty-four patients with
cardioembolism (CE) and ninety-four patients with small artery occlusion stroke (SAO),
and eighty-eight control subjects were evaluated. The lab parameters and levels of
PLA were measured within 3 h after the ischemic event in the case subjects and within empty stomach in the control
subjects. In all subjects, SELP S290N and PSGL-1 M62I polymorphisms were also measured.
Results
Platelet-monocyte aggregates (PMA), platelet-lymphocyte aggregates (PLyA) and platelet-neutrophil
aggregates (PNA) in LAA group, CE group and SAO group were all higher than that in
control group (P = 0.000 for all comparisons). Compared with LAA group, PMA and PLyA in CE group were
significantly higher (P = 0.018 and P = 0.003, respectively). Compared with SAO group, PMA and PLyA in CE group were also
significantly higher (P = 0.041 and P = 0.019, respectively). Compared with control group, there were significantly differences
of I allele frequencies of PSGL-1 M62I in LAA group and SAO group (OR = 2.249, 95%CI: 1.175–4.304, P = 0.021 and OR = 2.055, 95%CI: 1.269–3.328, P = 0.004, respectively). In SAO group, there were significantly differences of PNA among
MM, MI and II genotype of PSGL-1 M62I (P = 0.008).
Conclusions
PLA increased in AIS patients rapidly within 3 h. The I allele of PSGL-1 M62I was associated with risk of developing AIS, especially
LAA and SAO. SAO patients with the II genotype of PSGL-1 M62I have the higher level
of PNA.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of the Neurological SciencesAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Platelet function following acute cerebral ischemia.Angiology. 2009; 60: 362-369
- Course of platelet activation and platelet-leukocyte interaction in cerebrovascular ischemia.Stroke. 2006; 37: 2283-2287
- Platelet-leukocyte interactions link inflammatory and thromboembolic events in ischemic stroke.Ann. N. Y. Acad. Sci. 2010; 1207: 11-17
- Platelet-monocyte aggregates predict troponin rise after percutaneous coronary intervention and are inhibited by Abciximab.Int. J. Cardiol. 2005; 101: 249-255
- Modulation of P-selection and platelet aggregation in chronic periodontitis: a clinical study.J. Indian Soc. Periodontol. 2014; 18: 293-300
- Adhesive interaction between peripheral blood mononuclear cells and activated platelets in the presence of anti-human leukocyte antigen class I alloantibody causes production of IL-1b and IL-8.Vox Sang. 2012; 102: 250-257
- Molecular and functional interactions among monocytes, platelets, and endothelial cells and their relevance for cardiovascular diseases.J. Leukoc. Biol. 2009; 85: 195-204
- Polymorphism in the P-selectin and interleukin-4 genes as determinants of stroke: a population-based, prospective genetic analysis.Hum. Mol. Genet. 2004; 13: 389-396
- Association of the single-nucleotide polymorphism and haplotype of the P-selectin gene with ischemic stroke.J. Thromb. Thrombolysis. 2009; 27: 75-81
- Determinants of platelet-leukocyte aggregation and platelet activation in stroke.Cerebrovasc. Dis. 2015; 39: 176-180
- An updated definition of stroke for the 21st century: a statement for healthcare professionals from the American Heart Association/American Stroke Association.Stroke. 2013; 44: 2064-2089
- Classification of subtype of acute ischemic stroke. Definitions for use in a multicenter clinical trial. TOAST. Trial of org 10172 in acute stroke treatment.Stroke. 1993; 24: 35-41
- Differences in platelet-leukocyte aggregates among subtypes of acute cerebral ischemia.J. Neurol. Sci. 2011; 305: 126-130
- Contribution of SELP and PSGL-1 genotypes and haplotypes to the presence of coronary heart disease in Tunisians.Mol. Biol. Rep. 2010; 38: 495-501
- Acute cardioembolic cerebral infarction: answers to clinical questions.Curr. Cardiol. Rev. 2012; 8: 54-67
- Leukocyte-platelet aggregates in acute and subacute ischemic stroke.Cerebrovasc. Dis. 2009; 28: 276-282
- Platelet and monocyte activation in acute ischemic stroke-is there a correlation with stroke etiology?.Clin. Appl. Thromb. Hemost. 2012; 18: 87-91
- Platelet-leukocyte interaction and platelet activation in migraine: a link to ischemic stroke?.J. Neurol. Neurosurg. Psychiatry. 2004; 75: 984-987
- Platelet-leukocyte interaction and platelet activation in acute stroke with and without preceding infection.Arterioscler. Thromb. Vasc. Biol. 2005; 25: 1519-1523
- Levels and value of platelet activation markers in different subtypes of acute non-cardio-embolic ischemic stroke.Thromb. Res. 2009; 124: 213-218
- A comparative study of dual versus monoantiplatelet therapy in patients with acute large-artery atherosclerosis stroke.J. Stroke Cerebrovasc. Dis. 2014; 23: 1975-1981
- P-selectin gene haplotypes modulate soluble P-selectin concentrations and contribute to the risk of venous thromboembolism.Thromb. Haemost. 2008; 99: 899-904
- Specific P-selectin and P-selectin glycoprotein ligand-1 genotypes/haplotypes are associated with risk of incident CHD and ischemic stroke: the atherosclerosis risk in communities (ARIC) study.Atherosclerosis. 2007; 195: e76-e82
- Correlations of SELE and SELP genetic polymorphisms with myocardial infarction risk: a meta-analysis and meta-regression.Mol. Biol. Rep. 2014; 41: 4521-4532
- The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study.Mol. Biol. Rep. 2012; 39: 6479-6485
- The P-selectin gene is highly polymorphic: reduced frequency of the Pro715 allele carriers in patients with myocardial infarction.Hum. Mol. Genet. 1998; 7: 1277-1284
- Structure and function of the selectin ligand PSGL-1.Braz. J. Med. Biol. Res. 1999; 32: 519-528
- Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease.Clin. Chem. Lab. Med. 2004; 42: 997-1004
- Polymorphisms of P-selectin glycoprotein ligand-1 are associated with neutrophil-platelet adhesion and with ischaemic cerebrovascular disease.Br. J. Haematol. 2001; 115: 969-976
Article info
Publication history
Published online: May 24, 2016
Accepted:
May 23,
2016
Received in revised form:
May 9,
2016
Received:
November 12,
2015
Identification
Copyright
© 2016 Elsevier B.V. All rights reserved.
