- •The levels of PLA including PMA, PLyA and PNA and polymorphisms of SELP S290N and PSGL-1 M62I were measured.
- •The differences of PMA, PLyA and PNA among subtype patients were compared respectively.
- •We compared the genotypes and allele frequencies of SELP S290N and PSGL-1 M62I among subtype patients respectively.
- •The relationship between PLA and SELP S290N and PSGL-1 M62I polymorphisms was analyzed.
Platelet-leukocyte aggregations (PLA) play key roles in acute ischemic stroke (AIS) process and they are formed by the combination of P-selectin (SELP) expressed on the surface of the platelet membranes with P-selectin glycoprotein ligand-1(PSGL-1) expressed on the surface of the leukocytes. There are genetic polymorphisms in SELP and PSGL-1. We tested the differences in all kinds of PLA among subtypes of AIS and the association of SELP S290N and PSGL-1 M62I polymorphism with AIS to assess the correlations of PLA with SELP S290N and PSGL-1 M62I genetic polymorphisms.
One hundred and forty-eight patients with acute ischemic stroke, including thirty patients with large artery atherosclerosis stroke (LAA), twenty-four patients with cardioembolism (CE) and ninety-four patients with small artery occlusion stroke (SAO), and eighty-eight control subjects were evaluated. The lab parameters and levels of PLA were measured within 3 h after the ischemic event in the case subjects and within empty stomach in the control subjects. In all subjects, SELP S290N and PSGL-1 M62I polymorphisms were also measured.
Platelet-monocyte aggregates (PMA), platelet-lymphocyte aggregates (PLyA) and platelet-neutrophil aggregates (PNA) in LAA group, CE group and SAO group were all higher than that in control group (P = 0.000 for all comparisons). Compared with LAA group, PMA and PLyA in CE group were significantly higher (P = 0.018 and P = 0.003, respectively). Compared with SAO group, PMA and PLyA in CE group were also significantly higher (P = 0.041 and P = 0.019, respectively). Compared with control group, there were significantly differences of I allele frequencies of PSGL-1 M62I in LAA group and SAO group (OR = 2.249, 95%CI: 1.175–4.304, P = 0.021 and OR = 2.055, 95%CI: 1.269–3.328, P = 0.004, respectively). In SAO group, there were significantly differences of PNA among MM, MI and II genotype of PSGL-1 M62I (P = 0.008).
PLA increased in AIS patients rapidly within 3 h. The I allele of PSGL-1 M62I was associated with risk of developing AIS, especially LAA and SAO. SAO patients with the II genotype of PSGL-1 M62I have the higher level of PNA.
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Published online: May 24, 2016
Accepted: May 23, 2016
Received in revised form: May 9, 2016
Received: November 12, 2015
© 2016 Elsevier B.V. All rights reserved.