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The suppression of experimental autoimmune encephalomyelitis by intraperitoneal administration of HuangQi Glycoprotein

      Background: HuangQi Glycoprotein (HQGP) is a kind of natural plant glycoprotein derived from Astragalusm Embranaceus. In vitro experiments showed that HQGP can inhibit lymphocyte proliferation and immune responses and HuangQi has been applied in the treatment of multiple sclerosis (MS).
      Objective: To explore the therapeutic potential of HQGP in the experimental autoimmune encephalomyelitis (EAE), and elucidate its possible mechanism.
      Material and methods: Mouse EAE was induced by MOG35–55 immunization and treated intraperitoneally with HQGP once every day from day 3 post-immunization (p.i.) to day 27p.i. Mice that received the same volume of saline served as untreated EAE controls. The study was approved by the Ethics Committee of Shanxi University of Traditional Chinese Medicine.
      Results: The administration of HQGP delayed the onset of EAE, attenuated the clinical symptoms and inhibited inflammatory infiltration into the CNS. HQGP can inhibit the proliferation of mononuclear cells, suppress the secretion of TNF-α, IL-6, NO and promote the secretion of IFN-γ. The results further demonstrated that HQGP treatment effectively induced CD4+CD25+ T cell, CD4+IL-10+ T cell and CD4+IFN-γ+ T cell.
      Conclusions: The administration of HQGP exhibits therapeutic effect on the development of EAE and significantly reduces the inflammatory foci of CNS, possibly through regulating reactive T-cell homeostasis, suppressing the secretion of inflammatory cytokines and inhibiting neuroinflammation in the CNS. (Grant: National Natural Science Foundation of China, 81473577; Shanxi Scholarship Council of China, 2014-00023; The 2011 Cultivation Project of Shanxi University of Traditional Chinese Medicine, 2011PY-1).