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MiR-377 affects glioma cells proliferation, apoptosis, and invasion via targeted regulation of Ets-1

  • Author Footnotes
    1 The authors contributed equally to this work.
    Dong Wang
    Footnotes
    1 The authors contributed equally to this work.
    Affiliations
    Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China

    Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, Guangdong, China
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  • Author Footnotes
    1 The authors contributed equally to this work.
    Shao-qin Zheng
    Footnotes
    1 The authors contributed equally to this work.
    Affiliations
    Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China
    Search for articles by this author
  • Yong Zeng
    Affiliations
    Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China
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  • Hai-bin Chen
    Correspondence
    Corresponding authors at: 57# Changping Road, Shantou 515041, Guangdong Province, P.R. China.
    Affiliations
    Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China

    Department of Histology and Embryology, Shantou University Medical College, Shantou 515041, Guangdong, China
    Search for articles by this author
  • Ying-ming Yang
    Correspondence
    Corresponding authors at: 57# Changping Road, Shantou 515041, Guangdong Province, P.R. China.
    Affiliations
    Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China
    Search for articles by this author
  • Author Footnotes
    1 The authors contributed equally to this work.

      Highlights

      • Ets-1 expression in glioma tissues was significantly higher.
      • Ets-1 expression associated with the degree of tumor malignancy and positively correlated with Ki-67 expression.
      • The MVD value increased gradually with enhanced Ets-1 expression.
      • MiR-377 can target and regulate Ets-1.
      • miR-377 can inhibit glioma cell proliferation, promote apoptosis, and suppress invasion.

      Abstract

      Glioma is the most common and aggressive primary brain tumor in clinic. Due to its high heterogeneity and not-fully understood pathogenesis, the progress of clinical treatment of glioma is slow. Current studies have demonstrated that Ets-1 (E26 tansformation-specific-1) is a proto-oncogene. Our immunohistochemical analysis indicated that the expression level of Ets-1 in glioma tissues was significantly higher than that in normal brain tissues (P<0.05), and the expression levels of Ets-1 associated with the degree of tumor malignancy and positively correlated with Ki-67 expression. Detection also found that the microvessel density (MVD) value of CD34 in glioma tissue was significantly higher than in normal brain tissue (P<0.001), and the MVD value increased gradually with enhanced Ets-1 expression, suggesting a close relation between Ets-1 and tumor angiogenesis. Further study revealed that in glioma cells, miR-377 can target and regulate Ets-1, and increased expression of miR-377 significantly inhibited the expression of Ets-1. An upregulated expression of Ets-1 can significantly promote the proliferation, inhibit the apoptosis, and promote the invasion of glioma cells. However, increased miR-377 expression can inhibit glioma cell proliferation, promote apoptosis, and suppress invasion. When both miR-377 mimics and Ets-1-overexpressing vector were cotransfected into glioma cells, detection results showed that the proliferation, apoptosis, and invasion of the cells did not show significant changes. So that miR-377 has a tumor suppressor function and can exert its effect by inhibiting the tumor-promoting function of Ets-1 via targeted regulation of the expression of the latter.

      Graphical abstract

      Keywords

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