- •MS is considered as a chronic inflammatory disease of the central nervous system.
- •The both nuclear and mitochondrial genes are involved in MS development.
- •The mtDNA of complex I gene variations are involved in the progression of MS.
- •Relationship between mitochondrial complex I gene variations and MS.
Background and purpose
Multiple sclerosis (MS) is an autoimmune-mediated inflammatory and debilitating disease of the central nervous system. Several investigations have suggested that the mitochondrial DNA encoded subunits of complex I gene variations are involved in the progression of MS. In this study, we investigated the possible association between mitochondrial complex I gene variations and MS in a Filipino population.
Material and methods
A total of 300 individuals were included in the present study, two-hundred patients with MS clinical symptoms, and one-hundred healthy subjects without MS clinical features. We amplified target genes of mtDNA using polymerase chain reaction technique (PCR), and sequenced these to evaluate mitochondrial complex I gene variations.
We found nine variations (Nt 4216 T > C, Nt 5153 A > G, Nt 10142 C > T, Nt 11353 T > C, Nt 11935 T > C, Nt 12062 C > T, Nt 13042 G > A, Nt 13708 G > A and Nt 14179 G > A) in mtDNA-encoded complex I subunit genes. Our results showed that the prevalence of ND1, ND2, ND3, ND4 and ND5 gene variations was significantly higher in patients than in healthy controls (P < 0.0001). Whereas, the frequency of Nt 14179 G > A variation in ND6 gene was significantly higher in the control group compared with the patients (P < 0.0001).
Taken together our data supports a strongly positive association between mitochondrial complex I gene variations and MS pathogenesis in a Filipino population.
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Published online: July 28, 2014
Accepted: July 21, 2014
Received in revised form: July 20, 2014
Received: April 30, 2014
© 2014 Elsevier B.V. Published by Elsevier Inc. All rights reserved.