Abstract
Background & objectives
Analysis of the role of different alleles of human leukocyte antigen (HLA) in multiple
sclerosis (MS) patients is necessary in many populations and geographical areas. The
aim of the present study was to investigate the frequency of HLA-DRB1 genes and its
influence on susceptibility to MS, comparing with that in control group.
Design and setting
Two groups of case–control of multiple sclerosis patients referred to clinic at Khatam
hospitals were studied. The first group consisted of 73 multiple sclerosis patients
and the second group comprised 40 healthy volunteers with no known history of MS,
living in Tehran.
Patients and methods
The sample population consisted of 73 consecutive non-selected patients diagnosed
with MS according to the McDonald criteria (2010) at the outpatient clinic for multiple
sclerosis, 62 (85%) presented with RRMS and 11 (15%) with SPMS. The frequency of HLA-DRB1
alleles was determined in 73 MS patients (with age of 18–56) and 40 healthy subjects
in Iran. These consisted of 57 females and 16 males. HLA-DRB1 allele types were identified
by polymerase chain reaction products of 24 pair primers for low resolution SSP typing
(PCR-SSP).
Results
The HLA-DRB1* 11/15 genotype was detected highest (6 times) in patients compare to
normal control population (p-value 0.062), whereas the DRB1 4/11 genotype was detected
highest (4 times) in controls compare to MS patients (p-value 0.033). The data showed
that HLA-DRB1*03 is significantly more in patients compare to control normal people
(p-value 0.0021) as well as DRB1 14 and 16 are significantly more in control normal
people, compare to MS patients (p-values 0.0789 and 0.035).
Conclusion
Allele frequency among patients with positive history of multiple sclerosis disease
showed that DRB1 11 allele has a significantly low rate in MS patients with positive
history compare to other patients. In contrast DRB1 15 allele has a significantly
high rate in MS patients with positive history compare to other patients. The frequencies
of other alleles were not significantly different between the MS patients and the
control group. The frequency of the HLA-DRB1* 11/15 genotype detected in the present
study showed that this genotype is partially significant factor for MS susceptibility
and development in Iran.
Keywords
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Article info
Publication history
Published online: July 16, 2014
Accepted:
July 7,
2014
Received in revised form:
April 30,
2014
Received:
March 5,
2014
Identification
Copyright
© 2014 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
