Abstract
Parkinson disease (PD) is the most important movement disorder and about 50% of patients
develop dementia over the time. PD belongs to the group of Lewy body disorders. Alpha-synuclein
(AS) is the main component of Lewy bodies and its aggregation is a key event in the
pathogenesis of PD. Beta-synuclein (BS) inhibits AS aggregation in vitro and in vivo
and has been shown to interact directly with AS regulating its functionality and preventing
its oligomerization. Recently, we have described a molecular subgroup of DLB characterized
by the drastic BS reduction in cortical areas. In this study we have analyzed the
expression of two BS transcripts and the main AS transcript SNCA140, in frozen samples
of three brain areas, temporal cortex, caudate nucleus and pons, from patients with
PD and PDD in comparison with controls. Relative mRNA expression was determined by
real-time PCR with SybrGreen, neuron-specific-enolase as housekeeping gene and the
deltadeltaCt method. The most important difference in BS and AS mRNA expression between
PD and PDD was found in the caudate nucleus, where BS mRNA was overexpressed in PD
and AS mRNA diminished in PDD. Our findings provide new insights into the pathogenesis
of dementia in PD, indicating that differential BS and AS expression in the caudate
nucleus may represent one of the molecular mechanisms involved in these complex diseases.
Keywords
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Article info
Publication history
Published online: June 20, 2011
Accepted:
May 31,
2011
Received in revised form:
May 12,
2011
Received:
March 22,
2011
Identification
Copyright
© 2011 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
