Abstract
Brain energy disorders and oxidative stress due to chronic hypoperfusion are considered
to be major risk factors in the pathogenesis of dementia. The aim of our study was
to evaluate changes of the brain creatine kinase (BB-CK) reaction and mitochondrial
respiratory chain function in male Wistar rats exposed to chronic cerebral hypoperfusion.
Three-vessel occlusion (3-VO) was accomplished without thoracotomy using a minimally-invasive
surgical approach for the occlusion of the brachiocephalic trunk and the left common
carotid artery (CCA). The forward rate constant of creatine kinase (kfor) was measured in vivo by saturation transfer of 31P magnetic resonance spectroscopy (MRS) at 2 and 10 weeks of permanent 3-VO. The function
of the mitochondrial respiratory chain in vitro was assessed polarographically at 10 weeks after 3-VO. When compared to the controls,
the significant 42% reduction of kfor at 2 resp. 10 weeks indicated disorders in brain energy metabolism, which is in agreement
with the 12% decrease of the oxidative phosphorylation coefficient (ADP:O) and with
the 14% decrease of the oxidative phosphorylation rate (OPR) measured in isolated
mitochondria. Oxidative modification of the creatine kinase system (inactivation of
enzymes) and metabolic disorders due to chronic 3-VO, thus, may participate in vascular
cognitive impairment and neuronal degeneration.
Keywords
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Article info
Publication history
Published online: March 09, 2009
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© 2009 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
