Abstract
Background
Ischemic stroke is the end phenotype of a complex interaction between various genetic
and environmental factors.
Objective
We aimed to explore association of two lipid-relevant genetic variants and conventional
risk factors with risk of having ischemic stroke in Northern Han Chinese.
Methods
Genotyping was performed in 396 ischemic stroke patients and 396 controls that were
all recruited from the four hospitals of Qiqihar city. Data were analyzed using χ2 test, logistic regression and haplotype analyses.
Results
Significant differences were observed for genotype and allele distributions of APOE ε2/ε3/ε4 polymorphism (P<0.001) with ε4 allele conferring a 2.19-fold risky effect (P<0.001), while no statistical differences were found for LDLR C1773T distributions. Haplotype analysis indicated the remarkable differences for
haplotypes harboring APOE “ε2” or “ε4” alleles between cases and controls after the stringent Bonferroni correction.
Moreover, all multiple-testing associations remained significant using false discovery
rate (FDR) method. Further our multiple logistic regression analysis showed significant
associations of hypertension status (OR=5.37, P<0.001) and APOE ε2 (OR=0.45, P<0.001) and ε4 (OR=1.50, P=0.003) alleles with ischemic stroke after controlling confounders, and their correlations
with plasma lipid profiles were strengthened by stratification of alleles and hypertension
status combined.
Conclusions
Our results not only demonstrated potential interactions of APOE ε2/ε3/ε4 and LDLR C1773T polymorphisms with risk of having ischemic stroke, but also added the evidence
of independent role of hypertension and APOE ε2/ε3/ε4 polymorphism in the development of this disorder in Northern Han Chinese.
Keywords
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Article info
Publication history
Accepted:
September 12,
2008
Received in revised form:
September 2,
2008
Received:
July 4,
2008
Footnotes
☆Funding: Our work is supported by the Foundation for University Key Teacher by the Heilongjiang Ministry of Education and the Science and Technology Key Project of Heilongjiang (Grant No. G98L 19-22).
Identification
Copyright
© 2008 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
