Abstract
Aquaporin-4 (AQP4) is a water channel protein that plays an important role in water
movement in the central nervous system (CNS). Recently, presence of anti-AQP4 antibody
has been reported in the sera from patients with neuromyelitis optica. AQP4 is therefore
a possible target for inflammatory mechanisms in CNS. In the present investigation,
we performed semi-quantitative analysis of AQP4-mRNA in brain and spinal cord from
mice affected with experimental autoimmune encephalomyelitis (EAE) using real-time
PCR. AQP4-mRNA expression was increased in EAE; reaching a peak in the spinal cord
at 14 days, and in the brain at 21 days after first inoculation. Immunohistochemical
analysis showed that AQP4 is expressed on astrocytes, indicating that the increase
in AQP4 expression may correlate with astrocytic activation. This is the first study
to demonstrate upregulation of AQP4 in EAE. The upregulation of AQP4 could be involved
in the development of inflammation in the acute phase of EAE.
Keywords
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Article info
Publication history
Accepted:
September 11,
2008
Received in revised form:
August 26,
2008
Received:
January 18,
2008
Identification
Copyright
© 2008 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
