Abstract
The objective of the present study was to evaluate the neurotoxic effects of lindane,
in mice and the protective potential of two antioxidants alpha-lipoic acid (ALA) and
vitamin E, against the observed lindane induced toxicity. 7–8 weeks old healthy Swiss
mice were administered acute doses of lindane (40 mg/kg b.w.) or antioxidants or both
subcutaneously and analyzed 18 h later. ALA and vitamin E were used in the combination
for neuroprotection in the concentration of 20 mg/kg b.w. and 50 mg/kg b.w. respectively.
Lipid peroxidation, gamma-amino butyric acid (GABA) and serotonin level were used
as biochemical test of toxic action for lindane induced acute toxicity. Protective
effects of ALA and vitamin E were also evaluated on the same parameters. Reduced glutathione
(GSH) and thiobarbituric acid reactive substances (TBARS) level served as an index
for determining the extent of lipid peroxidation. Treatment of lindane to normal control
animals resulted in a significant decrease and increase in GSH (P<0.01) and TBARS level (P<0.01) respectively in crude homogenate of whole brain. Antioxidants treatment significantly
altered the level of GSH (P<0.01) and TBARS (P<0.01). GABA and serotonin level in whole brain as well as in different regions of
brain were measured. Main brain regions under the investigation were olfactory lobe,
cerebrum, hippocampus–hypothalamus, cerebellum and pons-medulla. Critical difference
(CD) of GABA level in various groups was found significant at 1% in cerebrum and hippocampus–hypothalamus,
at 5% in whole brain, cerebellum and pons-medulla (i.e. P<0.01 and P<0.05 respectively). Change in serotonin level in whole brain as well as in all studied
brain regions of various groups was found significant at 1% CD (i.e. P<0.01).
Keywords
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Article info
Publication history
Accepted:
September 11,
2008
Received in revised form:
September 6,
2008
Received:
June 12,
2007
Identification
Copyright
© 2008 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
