Abstract
Background
14-3-3 protein plays crucial roles in tumorigenesis, including the maintenance of
cell cycle and DNA repair, the prevention of apoptosis, among others. In mammalian
cells, seven 14-3-3 isoforms (β, ε, ζ, η, θ, γ and σ) have been identified and each
of these seems to have distinct tissue localizations and isoform-specific functions.
In the present study, the levels of all seven 14-3-3 isoforms were examined in astrocytoma.
Methods
The expression of 14-3-3 isoforms and their protein expression levels were examined
in five glioma cell lines by western blotting. Then in astrocytoma tissues, we investigated
expression percentages of each isoform by immunohistochemistry. The protein and mRNA
expression levels of each isoform were also detected by western blotting and RT-PCR,
respectively.
Results
14-3-3β and η were specifically expressed in astrocytoma, and their expression frequencies
and levels increased with the increase of astrocytoma malignancy. The result from
glioma cell lines was consistent with that from astrocytoma tissue.
Conclusions
In our study, we found two tumor-specific isoforms of 14-3-3 in astrocytoma. They
might be involved in astrocytoma tumorigenesis and may be useful as targets for therapy.
Keywords
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Article info
Publication history
Accepted:
August 26,
2008
Received in revised form:
August 20,
2008
Received:
April 12,
2008
Identification
Copyright
© 2008 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
