Abstract
PANDAS and some cases of Tourette syndrome (TS) have been proposed to be post-streptococcal
movement disorders in which antibodies produced against group A β-hemolytic streptococcus
cross react against brain epitopes. Attempts to identify disease specific anti-striatal
antibodies in the serum of affected patients have focused on the use of Western immunoblotting
and ELISA methodologies. In this study, immunohistochemical techniques were used to
identify serum anti-striatal antibody reactivity. In positive samples, double staining
with anti-GFAP (glial) and anti-MAP2 (neuronal) was used to establish localization
of the immunofluorescence. No significant differences in immunofluorescence or localization
were identified in patients with PANDAS (n=30) and TS (n=30) as compared to controls (n=30). IF reactivity did not correlate with tic severity or elevated titers of antistreptococcal
antibodies. Further comparisons showed no correlation between autoreactivity determined
by immunofluorescence and the presence of previously measured immunoblot reactivity
against human caudate or putative antigens (pyruvate kinase M1 and aldolase C). These
results confirm an inability to distinguish patient populations by antibody measurements
and raise further concerns about the presence of an autoimmune mechanism in PANDAS
and TS.
Keywords
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Article info
Publication history
Accepted:
August 21,
2008
Received in revised form:
July 21,
2008
Received:
May 8,
2008
Identification
Copyright
© 2008 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
