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Research article| Volume 119, ISSUE 1, P91-98, October 1993

Induction of experimental autoimmune encephalomyelitis in rats and immune response to myelin basic protein in lipid-bound form

DOI:https://doi.org/10.1016/0022-510X(93)90196-6
Induction of experimental autoimmune encephalomyelitis in rats and immune response to myelin basic protein in lipid-bound form
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      Abstract

      Encephalitogenic activity of myelin basic protein (MBP) isolated in a form retaining binding to all myelin lipids was tested in Lewis rats. Immunization with this new stable lipid-bound and native-like preparation (LB-MBP), induced experimental autoimmune encephalomyelitis (EAE) as intensively as the classical lipid free MBP (LF-MBP). During the course of the disease, high affinity specific response to LB-MBP and high frequency of LB-MBP specific precursors was observed in peripheral lymphoid organs, indicating that the disease occurred in presence of anti LB-MBP specific T-cell responsivity. Short term lines, generated from lymphocytes collected at the onset of the disease from LB-MBP immunized rats, showed a strong dose-dependent response to LB-MBP, but not to LF-MBP. The present data indicate that in rat, LB-MBP maintains encephalitogenic activity and induces expansion of a specific T-cell population. These data suggest also that LB-MBP is a new autoantigen that may be relevant in human diseases.

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      References

        • Alvord E.C.
        • Kies M.W.
        • Suckling A.L.
        Experimental Allergic Encephalomyelitis: a useful model for multiple sclerosis.
        Alan R. Liss Inc, New York1984
        • Brostoff S.W.
        Immunological responses to myelin and myelin components.
        in: Morell P. Myelin. Plenum Publishing Co, New York1984: 405-439
        • Cavatorta P.
        • Masotti L.
        • Szabo A.G.
        • Riccio P.
        • Quagliarello E.
        Structural dynamics of myelin basic protein in the assembly of a model membrane.
        in: Jameson D.M. Reinhart G. Fluorescent Biomolecules: Methodologies and Applications. Academic Press, New York1989: 383-388
        • Cohen J.A.
        • Essayan D.M.
        • Zweiman B.
        • Lisak R.P.
        Limiting dilution analysis of the frequency of antigen reactive lymphocytes isolated from the Central Nervous System of Lewis rats with experimental allergic encephalomyelitis.
        Cell. Immunol. 1987; 108: 203-213
        • Deibler G.E.
        • Martenson R.E.
        • Kies M.W.
        Large scale preparation of myelin basic protein from central nervous tissue of several mammalian species.
        Prep. Biochem. 1972; 2: 139-165
        • Deres K.
        • Schild H.
        • Wiesmuller K.H.
        • Jung G.
        • Rammensee
        In vivo priming of virus-specific cytotoxic T lymphocytes with synthetic lipopeptide vaccine.
        Nature. 1989; 342: 561-564
        • Lees M.B.
        • Brostoff S.W.
        Proteins of Myelin.
        in: Morell P. Myelin. Plenum Publishing Co, New York1984: 405-439
        • Liuzzi G.M.
        • Rizzo T.
        • Ventola A.
        • Riccio P.
        • Quagliarello E.
        Purification of lipid-associated basic protein from guinea pig spinal cord myelin.
        Acta Neurol. 1991; 2: 113-120
        • Lolli F.
        • Liuzzi G.M.
        • Vergelli M.
        • Massacesi L.
        • Ballerini C.
        • Amaducci L.
        • Riccio P.
        Antibodies specific for the lipid-bound form of myelin basic protein during experimental autoimmune encephalomyelitis.
        J. Neuroimmunol. 1993; (in press)
        • McFarlin D.E.
        • McFarland H.F.
        Multiple sclerosis.
        N. Engl. J. Med. 1982; 307: 1246-1251
        • Nair S.
        • Zhou F.
        • Reddy R.
        • Huang L.
        • Rouse B.T.
        Soluble proteins delivered to dendritic cells via pH-sensitive liposome induce primary cytotoxic T lymphocyte responses in vitro.
        J. Exp. Med. 1992; 175: 609-612
        • Riccio P.
        • Rosenbusch J.P.
        • Quagliariello E.
        A new procedure for the isolation of the brain myelin basic protein in a lipid-bound form.
        FEBS Lett. 1984; 177: 236-240
        • Riccio P.
        • Masotti L.
        • Cavatorta P.
        • De Santis A.
        • Juretic D.
        • Bobba A.
        • Pasquali-Ronchetti I.
        • Quagliariello E.
        Myelin basic protein ability to organize lipid bilayers: structural transition in bilayers of lysophosphatidylcholine micelles.
        Biochem. Biophys. Res. Commun. 1986; 134: 313-319
        • Riccio P.
        • Liuzzi G.M.
        • Quagliariello E.
        Lipid-bound, native-like, myelin basic protein: batch-wise preparation and perspectives for use in demyelinating diseases.
        Mol. Chem. Neuropathol. 1990; 13: 185-194
        • Schild H.
        • Deres K.
        • Wiesmuller K.H.
        • Jung G.
        • Rammensee H.G.
        Efficiency of peptides and lipopeptides for in vivo priming of virus-specific cytotoxic T cells.
        Eur. J. Immunol. 1991; 21: 2649-2654

      Article info

      Publication history

      Accepted: April 12, 1993
      Received in revised form: March 8, 1993
      Received: October 12, 1992

      Identification

      DOI: https://doi.org/10.1016/0022-510X(93)90196-6

      Copyright

      © 1993 Published by Elsevier Inc.

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