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Abstract
A double-label two-dimensional electrophoretic procedure has been used to search for
abnormal proteins in the serum and plasma of patients with definite multiple sclerosis.
The procedure possesses high resolving power and is particularly valuable in comparative
studies of complex mixtures of proteins since differences due to gel-to-gel variations
in protein mobilities are eliminated. Proteins present in serum and plasma at a concentration
of 5–10 μg/ml are detected routinely. No consistent differences were observed between
patients with multiple sclerosis and normal subjects in comparisons using pooled or
individual specimens of serum or plasma. The absence of consistent differences in
serum and plasma proteins between normal subjects and MS patients applied even when
the sensitivity of the procedure was increased several-fold and when the possibly
obscuring effect of albumin in the electrophoretic gels was eliminated. To our knowledge,
this study combines the most extensive and sensitive search for consistent abnormalities
in serum and plasma proteins in multiple sclerosis sera thus far reported.
Keywords
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References
- High-resolution two-dimensional electrophoretic mapping of plasma proteins.in: 2nd edition. The Plasma Proteins. Vol. 4. Academic Press, New York, NY1984: 221-270
- Abnormalities of serum and plasma components in patients with multiple sclerosis.Clin. Biochem. 1985; 18: 3-13
- Labeling of proteins by reductive methylation using sodium cyanoborohydride.J. Biol. Chem. 1979; 254: 4359-4365
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature (Lond.). 1970; 227: 680-685
- High resolution two-dimensional electrophoresis of proteins.J. Biol. Chem. 1975; 250: 4007-4021
- A double-label two-dimensional gel electrophoresis procedure specifically designed for serum or plasma protein analysis.Analyt. Biochem. 1986; (In press)
Article info
Publication history
Accepted:
September 15,
1986
Received:
August 26,
1986
Footnotes
☆This work was supported by grants from the National Multiple Sclerosis Society of New Zealand, the Ada F. Walker bequest from the Wellington Hospital Board and the New Zealand Neurological Foundation. T.T. Wheeler is a recipient of the W. & B. Miller Postgraduate Scholarship from the New Zealand Neurological Foundation.
Identification
Copyright
© 1987 Published by Elsevier Inc.
