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A double-label two-dimensional electrophoretic procedure has been used to search for abnormal proteins in the serum and plasma of patients with definite multiple sclerosis. The procedure possesses high resolving power and is particularly valuable in comparative studies of complex mixtures of proteins since differences due to gel-to-gel variations in protein mobilities are eliminated. Proteins present in serum and plasma at a concentration of 5–10 μg/ml are detected routinely. No consistent differences were observed between patients with multiple sclerosis and normal subjects in comparisons using pooled or individual specimens of serum or plasma. The absence of consistent differences in serum and plasma proteins between normal subjects and MS patients applied even when the sensitivity of the procedure was increased several-fold and when the possibly obscuring effect of albumin in the electrophoretic gels was eliminated. To our knowledge, this study combines the most extensive and sensitive search for consistent abnormalities in serum and plasma proteins in multiple sclerosis sera thus far reported.
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Accepted: September 15, 1986
Received: August 26, 1986
☆This work was supported by grants from the National Multiple Sclerosis Society of New Zealand, the Ada F. Walker bequest from the Wellington Hospital Board and the New Zealand Neurological Foundation. T.T. Wheeler is a recipient of the W. & B. Miller Postgraduate Scholarship from the New Zealand Neurological Foundation.
© 1987 Published by Elsevier Inc.