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Abstract
Paraproteins display a characteristic pattern of limited heterogeneity on isoelectric
focusing. In a group of 50 patients with various neurological disorders and paraproteinaemia
this pattern could be demonstrated in serum and cerebrospinal fluid simultaneously,
regardless of the form of nervous system involvement or of the function of the blood-cerebrospinal
fluid barrier. Although there were no qualitative differences in the pattern between
paraproteins from benign and malignant cases, a distinct quantitative difference was
found: Paraproteins from myeloma patients had significantly more subfractions extending
over a wider pH range than paraproteins from patients with benign monoclonal gammopathy.
Due to the high sensitivity of isoelectric focusing, more paraproteins could be detected
in cases where other electrophoretic methods including immunoelectrophoresis were
negative. A surprisingly high percentage of patients was found with benign monoclonal
gammopathy and vascular lesions of the central nervous system.
Keywords
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References
- One cell-one immunoglobulin — Origin of limited heterogeneity of myeloma proteins.Biochem. J. 1970; 116: 241-248
- Die pathogenetische Wertigkeit monoklonaler Immunglobuline für die Myelom-Neuropathie.in: Seitz D. Vogel P. Hämoblastosen - Zentrale Motorik - Iatrogene Schäden - Myositiden. Springer Verlag, Berlin1983: 400-405
- Rapid purification of antibodies to single band purity using recycling isoelectric focusing.Electrophoresis. 1982; 3: 284-288
- Microheterogeneity of immunoglobulin G from plasmocytomas — Identification of two types of IgG by isoelectric focusing.Z. Immun. Forsch. 1975; 150: 370-377
- Heterogeneity of monoclonal immunoglobulin-G proteins studied by isoelectric focusing.Clin. Chim. Acta. 1974; 54: 243-248
- Isoelectric patterns in IgG myeloma proteins — Possible biochemical and clinical inferences.in: Arbuthnott J.P. Beeley J.A. Isoelectric Focusing. Butterworth, London1975: 319-326
- Heterogeneity of myeloma proteins.J. Clin. Invest. 1963; 42: 111-123
- Paraproteinämische Hämoblastosen.in: Handbuch der Inneren Medizin. Vol. II/5. Springer Verlag, Berlin1974: 245-452
- Protein studies on cerebrospinal fluid and neurological symptoms in myelomatosis.Acta Neurol. Scand. 1969; 45: 1-17
- Discrete gammaglobulin (M-)components in serum.Acta Med. Scand. 1966;
- Paraproteins.in: Proc. Roy. Soc. Med.62. 1969: 773-776
- Isoelectric focusing of IgA and IgM in composite acrylamide-agarose gels.J. Immunol. Meth. 1980; 36: 315-324
- Herpes simplex-Encephalitis und Plasmocytom — Zufällige Koinzidenz oder kausale Verknüpfung?.Nervenarzt. 1984; 55: 86-89
- Bestimmung oligoclonaler IgG-Subfraktionen im Liquor und Serum.1982 (LKB Application Note)
- Microheterogeneity of serum myeloma immunoglobulins revealed by a technique of high resolution two-dimensional electrophoresis.Electrophoresis. 1980; 1: 82-89
- Plasma-cell dyscrasia and peripheral neuropathy with a monoclonal antibody to peripheral-nerve myelin.N. Engl. J. Med. 1980; 303: 618-621
- Peripheral neuropathy in patients with benign monoclonal gammopathy — A pilot study.J. Neurol. 1982; 228: 185-194
- Neurologische Befunde und Liquorveränderungen bei paraproteinämischen Hämoblastosen.in: Seitz D. Vogel P. Hämoblastosen - Zentrale Motorik - Iatrogene Schäden - Myositiden. Springer Verlag, Berlin1983: 386-389
- The discrimination between different blood-CSF barrier dysfunctions and inflammatory reactions of the CNS by a recent evaluation graph for the protein profile of cerebrospinal fluid.J. Neurol. 1980; 224: 89-99
- Isoelectric focusing of CSF and serum proteins in neurological disorders combined with benign and malignant proliferations of reticulocytes and plasmocytes.J. Neurol. 1977; 216: 251-264
- Polyacrylamide-gel disc electrophoresis of native cerebrospinal fluid proteins with special reference to immunoglobulins and some clinical applications.J. Neurol. Sci. 1976; 29: 213-239
- Gel isoelectric focusing and IgG index for demonstration of intrathecal IgG synthesis in neurological disorders.Ann. Clin. Biochem. 1981; 18: 153-157
- Cerebrospinal fluid paraproteins in multiple myeloma.J. Lab. Clin. Med. 1965; 66: 280-293
- Isoelectric focusing of immunoglobulins.in: 3rd edition. Handbook of Experimental Immunology. Blackwell, London1978: 9.1-9.30
Article info
Publication history
Accepted:
February 10,
1984
Received in revised form:
January 30,
1984
Received:
September 1,
1983
Identification
Copyright
© 1984 Published by Elsevier Inc.
