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Abstract
Experiments have been performed to determine if fibroblasts from patients with Duchenne
muscular dystrophy (DMD) are defective in a process of membrane repair. Normal and
DMD fibroblasts were treated with phospholipase C from Clostridium perfringens to degrade plasma membrane phosphatidylcholine, and then phosphatidylcholine synthesis
was measured as the incorporation of [3H]choline into lipid. Phosphatidylcholine synthesis was stimulated by phospholipase
C treatment to a similar extent in normal and DMD fibroblasts. The activity of CTP:
phosphocholine cytidylyltransferase, the enzyme regulating phosphatidylcholine synthesis
in phospholipase C-treated mammalian cells, was also stimulated to the same extent
in both cell types. The subcellular location of the cytidylyltransferase was changed
by phospholipase C treatment from mostly cytosolic to mostly particulate in both normal
and DMD fibroblasts. It appears, therefore, that at least one type of membrane repair
system functions normally in DMD fibroblasts.
Keywords
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Article info
Publication history
Accepted:
January 27,
1984
Received:
October 27,
1983
Footnotes
☆This research was supported in part by a grant from the Muscular Dystrophy Association. E.M. was supported by a scholarship from the Minority Access to Research Careers Program, National Institutes of Health.
☆This is paper number 9661 from the Purdue Agricultural Experiment Station.
Identification
Copyright
© 1984 Published by Elsevier Inc.
