Research Article| Volume 54, ISSUE 2, P317-323, May 1982

A technique for microinjection of peripheral nerve

  • M.N. Ghabriel
    Address correspondence to: M. N. Ghabriel M. B., B. Ch., Ph. D. Department of Anatomy, Charing Cross Hospital Medical School, Fulham Palace Road, London W6 8RF, Great Britain.
    Department of Anatomy, Charing Cross Hospital Medical School, London W6 8RF, Great Britain
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  • G. Allt
    Department of Anatomy and Reta Lila Weston Institute of Neurological Studies, Middlesex Hospital Medical School, London WIP 6DB Great Britain
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      Microinjection of peripheral nerves has proved especially useful in experimental studies in demyelination and neurotoxicology. A technique is described here for inducing a pure circumscribed demyelinating lesion in a peripheral nerve. Lysophosphatidyl choline (2 μl of 1%) is injected through a glass micropipette (3–10 μm tip) with the aid of a micromanipulator.
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