Research Article| Volume 54, ISSUE 2, P279-285, May 1982

The blood-brain barrier in rats fed on diets high or low in saturated/unsaturated fat ratio

      This paper is only available as a PDF. To read, Please Download here.


      The product of the permeability × vascular surface rate area (PA) of the blood-brain barrier to [14C]sucrose has been measured in rats raised on synthetic diets in which the saturated/unsaturated fat constitution was controlled at high or low levels. Gas-liquid chromatography demonstrated marked differences in brain fatty acid constitution between the dietary groups.
      No statistically significant difference was found between the permeability measurements in rats maintained on any of the synthetic diets. nor was there any difference from rats raised on a standard laboratory pellet food.
      The opportunity was taken to look at 3 other properties of brain that might be affected by lipid constitution. There was no change in the form of the membranous intracellular inclusions that can be induced by intracerebral injections of suramin, and Fink-Heimer staining of degenerating axons, which is inhibited by fat extraction, worked equally well on each diet group. The sleep time after an anaesthetic injection of alcohol was not significantly changed.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Journal of the Neurological Sciences
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Anker R.L.
        • Cragg B.G.
        Development of the extrinsic connection of the visual cortex in the cat.
        J. comp. Neurol. 1974; 154: 29-42
        • Clausen J.
        • Moller J.
        Allergic encephalomyelitis induced by brain antigen after deficiency in polyunsaturated fatty acids during myelination.
        Acta neurol. scand. 1967; 43: 375-388
        • Cragg B.G.
        • Phillips S.C.
        Natural variation in the blood-brain barrier.
        Neurosci. Letters. 1982; 27: 309-312
        • Cutherbertson W.F.J.
        Nutrient requirement of rats and mice.
        in: Proc. Nutr. Soc. Eng. Scot.16. 1957: 70-76
        • Eddy D.E.
        • Harman D.
        Rat brain fatty acid composition - Effect of dietary fat and age.
        J. Gerontol. 1975; 30: 647-654
        • Evans D.H.L.
        • Hamlyn L.H.
        A study of silver impregnation methods in the central nervous system.
        J. Anat. (Lond.). 1956; 90: 193-203
        • Fink R.P.
        • Heimer L.
        Two methods for selective silver impregnation of degenerating axons and their synaptic endings in the central nervous system.
        Brain Res. 1967; 4: 369-374
        • Galli C.
        • Spagnudo C.
        • Agradi E.
        • Paoletti R.
        Comparative effects of olive oil and other edible fats on brain structural lipids during development.
        in: Lipids. Vol. 1. Raven Press, New York, New York1976
        • Gray G.M.
        Intestinal disaccharidase deficiencies and glucose-galactose malabsorption.
        in: Stanbury J.B. Wijngaarden J.B. Fredrickson D.S. The Metabolic Basis of Inherited Disease. 4th edition. McGraw-Hill, New York1978: 1526-1536
        • Guillery R.W.
        • Shirra B.
        • Webster K.E.
        Differential impregnation of degenerating nerve fibres in paraffin-embedded material.
        Stain Technol. 1961; 36: 9-13
        • Hers H.G.
        The concept of inborn lysosomal disease.
        in: Hers H.G. Van Hoof F. Lysosomes and Storage Diseases. Academic Press, New York1973: 147-171
        • Koblin D.D.
        • Deady J.E.
        Sensitivity to alcohol in mice with an altered brain fatty acid composition.
        Life Sci. 1981; 28: 1889-1896
        • Lamptey M.S.
        • Walker B.L.
        A possible essential role for dietary linolenic acid in the development of the young rat.
        J. Nutr. 1976; 106: 86-93
        • Mohrhauer H.
        • Holman R.T.
        Alteration of the fatty acid composition of brain lipids by varying levels of dietary essential fatty acids.
        J. Neurochem. 1963; 10: 523-530
        • Nauta W.J.H.
        • Gygax P.A.
        Silver impregnation of degenerating axon terminals in the central nervous system: (1) Technic, (2) Chemical notes.
        Stain Technol. 1951; 26: 5-11
        • Ohno K.
        • Pettigrew K.D.
        • Rapoport S.I.
        Lower limits of cerebrovascular permeability to nonelectrolytes in the conscious rat.
        Amer. J. Physiol. 1978; 235: 299-307
        • Pazzagli A.
        • Arnetoli G.
        • Pepeu I.
        • Amaducci L.A.
        Fatty acid changes in cerebrospinal fluid in neurological disorders as an index of changes in the blood-brain barrier.
        Neurology (Minneap.). 1970; 20: 783-786
        • Phillips S.C.
        Does ethanol damage the blood-brain barrier?.
        J. neurol. Sci. 1981; 50: 81-87
        • Rees S.
        Membranous neuronal and neuroglial inclusions produced by intracerebral injection of suramin.
        J. neurol. Sci. 1978; 36: 97-109
        • Seidel D.
        • Heipertz R.
        • Weisner B.
        Cerebrospinal lipids in demyelinating disease, Part 1 (Linoleic acid as an index of impaired blood-CSF barrier).
        J. Neurol. 1980; 222: 177-182
        • Sinclair A.J.
        • Crawford M.A.
        Ultrastructural changes in the cerebrovascular endothelium induced by a diet high in linoleic acid and deficient in vitamin E.
        Exp. mol. Path. 1973; 21: 289-299
        • Sinclair A.J.
        • McLean J.G.
        • Monger E.A.
        Metabolism of linoleic acid in the cat.
        Lipids. 1979; 14: 932-936