This paper is only available as a PDF. To read, Please Download here.
JC virus (JCV)-induced T antigen developed in the nuclei of approximately 50% of adult human brain (AHB) cells infected with JCV. Approximately 10% of these T antigen-positive cells contained glial fibrillary acidic protein (GFAP) and were identified as astrocytes in immunofluorescence studies. About 50% of these T antigen-positive cells bound antiserum to fibronectin on their surface and were identified as fibroblasts. No cells binding antiserum to galactocerebroside (GC), an oligodendrocyte-specific marker, were detected in infected or non-infected AHB cultures. The cell type of AHB that constitutes the remaining 40–50% of T antigen-positive cells that are GFAP-, fibronectin- and GC-negative remains to be determined.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Journal of the Neurological Sciences
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Localization of glial fibrillary acidic protein in astrocytes by immunofluorescence.Brain Res. 1972; 43: 429-435
- An acidic protein isolated from fibrous astrocytes.Brain Res. 1971; 23: 351-354
- Human brain tissue in culture, Part 1 (Acquisition, initial processing, and establishment of brain cell cultures).J. comp. Neurol. 1975; 161: 295-306
- Human brain in tissue culture, Part 5 (Identification of glial cells by immunofluorescence).J. neurol. Sci. 1976; 29: 177-184
- Fluorescent Antibody Methods.Academic Press, New York, NY1969
- Cell type specific markers for human glia and neuronal cells in culture.Lab. Invest. 1980; 43: 342-358
- New human papovaviruses.in: Progress in Medicine and Virology. Vol. 22. Karger, Basel1976: 1-5
- JC papovavirus in progressive multifocal leukoencephalopathy.J. infect. Dis. 1976; 133: 686-690
- Galactocerebroside is a specific cell surface antigenic marker for oligodendrocytes in culture.Nature (Lond.). 1978; 274: 813-816
- Human brain in culture, Part 4 (Morphological characteristics).J. comp. Neurol. 1975; 161: 329-340
- JC human papovavirus replication in human amnion cells.J. Virol. 1979; 30: 384-389
- Immunological study of the glial fibrillary acidic protein.Brain Res. 1972; 37: 81-89
- A common cell-type specific surface antigen in cultured human glial cells and fibroblasts — Loss in malignant cells.J. exp. Med. 1976; 143: 64-72
- Distribution of fibroblast surface antigen — Association with fibrillar structures of normal cells and loss upon viral transformation.J. exp. Med. 1974; 140: 1522-1533
- Human brain in tissue culture, Part 2 (Studies of long-term cultures).J. comp. Neurol. 1975; 161: 307-316
- Growth of JC virus in adult human brain cell cultures.Arch. Virol. 1980; 65: 141-148
Accepted: October 21, 1981
Received in revised form: August 18, 1981
Received: June 12, 1981
☆This study was supported in part by Public Health Service Grant NS-11036 from the National Institutes of Health and by grant RG-851B5 from the National Multiple Sclerosis Society.
© 1982 Published by Elsevier Inc.