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Research Article| Volume 47, ISSUE 1, P135-144, July 1980

Characterization of membrane markers of isolated oligodendrocytes and clonal lines of the nervous system

DOI:https://doi.org/10.1016/0022-510X(80)90032-5
Characterization of membrane markers of isolated oligodendrocytes and clonal lines of the nervous system
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      Abstract

      We show here that the major glycolipid in myelin, galactocerebroside, is not only a useful marker for isolated bovine oligodendrocytes but that its quantitation can serve as a probe for cell differentiation. We have produced in the rabbit antisera to bovine oligodendroglia, bovine myelin and galactocerebroside. The binding of these antisera to membrane components of bovine oligodendroglia, bovine thymocytes, mouse glioma (oligodendroglioma) clonal cell strain G 26-24 and mouse neuroblastoma NB2 A was investigated with [125I]protein A. We found major differences in immunological properties between the mature oligodendrocyte and clonal lines of oligodendroglioma cells. Differentiation appears to imply not only the expression of galactocerebroside but also of additional surface markers that are absent from the neurotumor cell lines.
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      Article info

      Publication history

      Accepted: February 13, 1980
      Received: January 3, 1980

      Footnotes

      ☆This work was supported by the Swiss Multiple Sclerosis Society and the Swiss National Fund.

      Identification

      DOI: https://doi.org/10.1016/0022-510X(80)90032-5

      Copyright

      © 1980 Published by Elsevier Inc.

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