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Abstract
The first two enzymes of the pentose-phosphate pathway (G6PD and 6PGD) were studied
in rabbit skeletal muscle. Their activities closely paralleled the connective tissue
levels in each individual muscle, and they increased in atrophying muscles at the
same rate as connective tissue. Skeletal muscle tendons and subcutaneously implanted
polyvinyl sponges, both of which consist of relatively pure connective tissue, displayed
much higher activity of these enzymes than the muscle homogenates. These results suggest
that pentose synthesis in muscle is mostly confined to its connective tissue elements,
raising the possibility of a role for connective tissue in the biosynthetic processes
of skeletal muscle.
The contribution of connective tissue to the results of muscle quantitative studies
may be significant, and even overshadow other values in atrophic muscles, where the
muscle cell population may be greatly reduced. The interpretation of quantitative
biochemical studies on pathological muscle samples should take this factor into consideration.
Abbreviations:
G6PD (glucose-6-phosphate dehydrogenase), 6PGD (6-phosphogluconate dehydrogenase), NCP (non-collagenous protein), DNA (deoxyribonucleic acid), RNA (ribonucleic acid), NADP+ (nicotinamide adenine dinucleotide phosphate oxidized form), NADPH (nicotinamide adenine dinucleotide phosphate reduced form)To read this article in full you will need to make a payment
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Article info
Publication history
Accepted:
February 20,
1980
Received:
December 4,
1979
Footnotes
☆This investigation was supported in part by grant NS-07786 from the National Institute of Health.
Identification
Copyright
© 1980 Published by Elsevier Inc.
