Difference of fibroblast growth factor receptor 1 expression among CA1-3 regions of the gerbil hippocampus after transient cerebral ischemia

Yoo, Ki-Yeon; Hwang, In Koo; Lee, Choong Hyun; Choi, Jung Hoon; Kwon, Seung-Hae; Kang, Il-Jun; You, Sang Guan; Kim, Young-Myeong; Won, Moo-Ho

doi:10.1016/j.jns.2010.06.018

« Previous Next »Journal of the Neurological Sciences
Volume 296, Issue 1 , Pages 13-21, 15 September 2010

Difference of fibroblast growth factor receptor 1 expression among CA1-3 regions of the gerbil hippocampus after transient cerebral ischemia

Ki-Yeon Yoo
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
In Koo Hwang
- Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Choong Hyun Lee
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Jung Hoon Choi
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Seung-Hae Kwon
- Division of Analytical Bio-imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, 200-701, Republic of Korea
Il-Jun Kang
- Department of Food Science and Nutrition, Hallym University, Chuncheon 200-702, Republic of Korea
- Corresponding authors. Won is to be contacted at the Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea. Tel.: +82 33 248 2522; fax: +82 33 256 1614. Kang, Tel.: +82 33 248 2135; fax: +82 33 255 4787.
Sang Guan You
- Marine Food Science and Technology, Kangnung-Wonju National University, Gangneung 210-702, Republic of Korea
Young-Myeong Kim
- Vascular System Research Center and Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea
Moo-Ho Won
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
- Corresponding authors. Won is to be contacted at the Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea. Tel.: +82 33 248 2522; fax: +82 33 256 1614. Kang, Tel.: +82 33 248 2135; fax: +82 33 255 4787.

Received 11 January 2010; received in revised form 16 June 2010; accepted 17 June 2010. published online 12 July 2010.

Abstract

Fibroblast growth factors are important regulators of neuronal development. In this study, we observed fibroblast growth factor receptor 1 (FGFR1) immunoreactivity and its protein levels in the hippocampus proper (CA1-3 regions) of the gerbil at various time points after ischemia/reperfusion. In the sham-operated group, FGFR1 immunoreaction was not detected in the hippocampus proper. FGFR1 immunoreaction was first detected in non-pyramidal neurons in the CA1-3 region at 12h and 1day after ischemia/reperfusion. From 2days after ischemia/reperfusion, FGFR1 immunoreaction was found in astrocytes, not in microglial cells, in the CA1 region: FGFR1 immunoreactivity and the number of astrocytes were significantly increased at 5days post-ischemia. Western blot analysis revealed that FGFR1 protein levels were also increased from 1day after ischemia/reperfusion. These results indicate that increase of FGFR1 in astrocytes of the ischemic CA1 region may be associated with gliosis followed by delayed neuronal death.