Maintenance of anti-inflammatory cytokines and reduction of glial activation in the ischemic hippocampal CA1 region preconditioned with lipopolysaccharide

Yu, Jia Tian; Lee, Choong Hyun; Yoo, Ki-Yeon; Choi, Jung Hoon; Li, Hua; Park, Ok Kyu; Yan, Bingchun; Hwang, In Koo; Kwon, Young-Guen; Kim, Young-Myeong; Won, Moo-Ho

doi:10.1016/j.jns.2010.06.004

« Previous Next »Journal of the Neurological Sciences
Volume 296, Issue 1 , Pages 69-78, 15 September 2010

Maintenance of anti-inflammatory cytokines and reduction of glial activation in the ischemic hippocampal CA1 region preconditioned with lipopolysaccharide

Jia Tian Yu
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
- Department of Human Anatomy, Xuzhou Medical College, Xuzhou 221004, China
- Jia Tian Yu and Choong Hyun Lee contribute equally to this article.
Choong Hyun Lee
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
- Jia Tian Yu and Choong Hyun Lee contribute equally to this article.
Ki-Yeon Yoo
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Jung Hoon Choi
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Hua Li
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Ok Kyu Park
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
Bingchun Yan
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
In Koo Hwang
- Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Young-Guen Kwon
- Department of Biochemistry, College of Sciences, Yonsei University, Seoul 120-749, Republic of Korea
Young-Myeong Kim
- Vascular System Research Center and Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea
Moo-Ho Won
- Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea
- MRC Research Institute, Hallym University, Chuncheon 200-702, Republic of Korea
- Corresponding author. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea. Tel.: +82 33 248 2522; fax: +82 33 256 1614.

Received 5 March 2010; received in revised form 27 May 2010; accepted 2 June 2010. published online 28 June 2010.

Abstract

Lipopolysaccharide (LPS) induces a strong immune response, and pretreatment with low dose of LPS suppresses the production of proinflammatory mediators. In the present study, we investigated the effect of LPS preconditioning on the delayed neuronal death in the gerbil hippocampal CA1 region after 5min of transient cerebral ischemia. LPS preconditioning showed neuroprotective effects against ischemic damage in the hippocampal CA1 region after ischemic insult: About 92% of neurons in the CA1 region survived in the LPS-treated ischemia group. LPS preconditioning maintained anti-inflammatory cytokines, such as interleukin (IL)-4 and IL-13, in pyramidal neurons in the CA1 region after ischemia/reperfusion. In addition, IL-4 and IL-13 protein levels in the CA1 region of the LPS-treated ischemia group were similar to the vehicle-treated sham group. We found that reactive gliosis was markedly attenuated in the CA1 region of the LPS-treated ischemia group compared to the vehicle-treated ischemia group using immunohistochemistry of glial fibrillary acidic protein for astrocytes, and ionized calcium-binding adapter molecule 1 and isolectin B4 for microglia. These results indicate that LPS preconditioning may provide neuroprotection in the ischemic hippocampal CA1 region via maintenance of anti-inflammatory cytokines and suppression of glial activation.

Keywords: Lipopolysaccharide, Ischemia, Hippocampus, Neuroprotection, Anti-inflammatory cytokine, Glial activation